Background: NAD(P)H:quinone oxidoreductase (NQO1) is a flavoprotein that catalyzes two-electron reduction and\ndetoxification of quinones and its derivatives. NQO1 catalyzes reactions that have a protective effect against redox\ncycling, oxidative stress and neoplasia. High expression of NQO1 is associated with many solid tumors including\nthose affecting the colon, breast and pancreas; however, its role in the progression of ovarian carcinoma is largely\nundefined. This study aimed to investigate the clinicopathological significance of high NQO1 expression in serous\novarian carcinoma.\nMethods: NQO1 protein expression was assessed using immunohistochemical (IHC) staining in 160 patients with\nserous ovarian carcinoma, 62 patients with ovarian borderline tumors and 53 patients with benign ovarian tumors.\nQuantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect NQO1 mRNA expression levels.\nThe correlation between high NQO1 expression and clinicopathological features of ovarian carcinoma was evaluated\nby Chi-square and Fisher�s exact test. Overall survival (OS) rates of all of ovarian carcinoma patients were calculated\nusing the Kaplan-Meier method, and univariate and multivariate analyses were performed using the Cox proportional\nhazards regression model.\nResults: NQO1 protein expression in ovarian carcinoma cells was predominantly cytoplasmic. Strong, positive expression\nof NQO1 protein was observed in 63.8% (102/160) of ovarian carcinomas, which was significantly higher than in\nborderline serous tumors (32.3%, 20/62) or benign serous tumors (11.3%, 6/53). Importantly, the rate of strong,\npositive NQO1 expression in borderline serous tumors was also higher than in benign serous tumors. High expression\nof NQO1 protein was closely associated with higher histological grade, advanced clinical stage and lower OS rates in\novarian carcinomas. Moreover, multivariate analysis indicated that NQO1 was a significant independent prognostic\nfactor, in addition to clinical stage, in patients with ovarian carcinoma.\nConclusions: NQO1 is frequently upregulated in ovarian carcinoma. High expressin of NQO1 protein may be an effective\nbiomarker for poor prognostic evaluation of patients with serous ovarian carcinomas.
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